digitonin wash buffer (Thermo Fisher)
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Thermo Fisher
digitonin wash buffer
Digitonin Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/digitonin+buffer/Digitonin/bio_rxiv__64898__2026__05__05__722861-322-8-22
Average 97 stars, based on 1 article reviews
Digitonin Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/digitonin+buffer/Digitonin/bio_rxiv__64898__2026__05__05__722861-322-8-22
Average 97 stars, based on 1 article reviews
digitonin wash buffer - by Bioz Stars,
2026-09
97/100 stars
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Incubation:Article Title: KMT2C Loss Promotes NF2-Wildtype Meningioma Progression and Ferroptosis Sensitivity via Epigenetic Repression of Hippo Signaling. Article Snippet: .. The CUT&Tag Assay (Vazyme #TD904) was performed using 1 × 105 cells permeabilized with Article Title: KMT2C Loss Promotes NF2‐Wildtype Meningioma Progression and Ferroptosis Sensitivity via Epigenetic Repression of Hippo Signaling Article Snippet: .. The CUT&Tag Assay (Vazyme #TD904) was performed using 1 × 10 5 cells permeabilized with Article Title: Heat Shock Factor 1 Governs Sleep-Wake Cycles Across Species Article Snippet: The ConA beads-nuclei mixture was resuspended in cold Antibody Buffer: 20mM HEPES (Gibco), 150mM Sodium chloride (S5150, Sigma-Aldrich), 0.5mM Spermidine (Sigma-Aldrich), 1X Halt Protease Inhibitor Cocktail (Fisher Scientific), 0.05% Digitonin (300410, Millipore Sigma), 2mM EDTA (15575020, Invitrogen) and 2% Bovine Serum Albumin (Sigma-Aldrich), and incubated with 0.5μg of anti-HSF1 antibody (4356S, Cell Signaling), SNAP-ChIP Certified H3K4me3 Positive Control Antibody (MA511198, Thermo Fisher) or Rabbit IgG Negative Control Antibody (130042, EpiCypher) per 5x10 nuclei at 4°C overnight on nutator. .. After washing twice in cold Article Title: Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1 Article Snippet: After incubation, the beads were resuspended in a cold antibody buffer (2 mM EDTA in digitonin buffer) containing antibody (5 μg of Yy1 (D5D9Z) rabbit monoclonal antibody 46395, 2 μg of FLAG antibody (Sigma-Aldrich, F3165-.2MG), 5 μg of Rad21 (BIOZOL, GTX106012) and 1 μg of H3k27Ac (Abcam, 39133)), and the mixture was incubated on a nutator overnight at 4 °C. .. On the next day, the beads were washed twice and resuspended in 0.75 μl of pAG-Mnase in Centrifugation:Article Title: Deoxyarbutin targets mitochondria to trigger p53-dependent senescence of glioblastoma cells. Article Snippet: Cellular senescence is a natural barrier of the transition from premalignant cells to invasive cancer.. Pharmacological induction of senescence has been proposed as a possible anticancer strategy.. In this study, we found that deoxyarbutin inhibited the growth of glioblastoma (GBM) cells by inducing cellular senescence, independent of tyrosinase expression. Protease Inhibitor:Article Title: Heat Shock Factor 1 Governs Sleep-Wake Cycles Across Species Article Snippet: The ConA beads-nuclei mixture was resuspended in cold Antibody Buffer: 20mM HEPES (Gibco), 150mM Sodium chloride (S5150, Sigma-Aldrich), 0.5mM Spermidine (Sigma-Aldrich), 1X Halt Protease Inhibitor Cocktail (Fisher Scientific), 0.05% Digitonin (300410, Millipore Sigma), 2mM EDTA (15575020, Invitrogen) and 2% Bovine Serum Albumin (Sigma-Aldrich), and incubated with 0.5μg of anti-HSF1 antibody (4356S, Cell Signaling), SNAP-ChIP Certified H3K4me3 Positive Control Antibody (MA511198, Thermo Fisher) or Rabbit IgG Negative Control Antibody (130042, EpiCypher) per 5x10 nuclei at 4°C overnight on nutator. .. After washing twice in cold Binding Assay:Article Title: Heat Shock Factor 1 Governs Sleep-Wake Cycles Across Species Article Snippet: The ConA beads-nuclei mixture was resuspended in cold Antibody Buffer: 20mM HEPES (Gibco), 150mM Sodium chloride (S5150, Sigma-Aldrich), 0.5mM Spermidine (Sigma-Aldrich), 1X Halt Protease Inhibitor Cocktail (Fisher Scientific), 0.05% Digitonin (300410, Millipore Sigma), 2mM EDTA (15575020, Invitrogen) and 2% Bovine Serum Albumin (Sigma-Aldrich), and incubated with 0.5μg of anti-HSF1 antibody (4356S, Cell Signaling), SNAP-ChIP Certified H3K4me3 Positive Control Antibody (MA511198, Thermo Fisher) or Rabbit IgG Negative Control Antibody (130042, EpiCypher) per 5x10 nuclei at 4°C overnight on nutator. .. After washing twice in cold Control:Article Title: UFMylation of Pyruvate Dehydrogenase Regulates Mitochondrial Metabolism Article Snippet: .. Control, ΔUFM1 and ΔUFSP2 cells were lysed on ice in other:Article Title: Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1. Article Snippet: Phase contrast images and fluorescent images (GFP) were captured every 20 min and 4 h, respectively, with a ×10 phase contrast objective (Zeiss) and an AxioCam HRm camera. |